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invitrogen/AM1348/AM1344/AM1340现货
  • 发布日期:2017-12-29      浏览次数:353
    • ThermoFisherAM1340mMESSAGE mMACHINE® SP6 Transcription Kit
      ThermoFisherAM1344mMESSAGE mMACHINE® T7 Transcription Kit
      ThermoFisherAM1348mMESSAGE mMACHINE® T3 Transcription Kit

      invitrogen/AM1348/AM1344/AM1340现货

      订货找蚂蚁淘就对了,4000-520-626

      描述

      mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its structure precludes its incorporation at any other position in the RNA molecule.

      How mMESSAGE mMACHINE® kits work
      mMESSAGE mMACHINE® kits have a simplified reaction format in which all four ribonucleotides and cap analog are mixed in a single solution. The cap analog:GTP ratio of this solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts. mMESSAGE mMACHINE® kits are ideal for the routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications. The high yields are achieved by optimizing reaction conditions for RNA synthesis in the presence of high nucleotide concentrations. In addition, the RNA synthesized is protected from degradation by any contaminating ribonucleases that may be present with RNase inhibitor—a component of the Enzyme Mix.

      Using mMESSAGE mMACHINE® Kits
      In a 20 µL reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield large mass amounts of capped RNA. Up to 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 µg) and proportion of transcripts that are capped (80%). mMESSAGE mMACHINE® Kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE® kits are only optimized for use with the polymerases included in the kit. Using a different polymerase may result in low yields. The mMESSAGE mMACHINE® kits contain all the buffers and reagents necessary for 25 transcription reactions. Using the control template supplied with the kits (Xenopus elongation factor 1α, pTRI Xef), each mMESSAGE mMACHINE® reaction will yield approximay 20–30 µg of RNA using T3 or T7 RNA polymerase, or about 15–25 µg RNA using SP6 RNA polymerase.

      For Research Use Only。 Not for use in diagnostic procedures。

      invitrogen/AM1348/AM1344/AM1340现货

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      蚂蚁淘常见平行进口产品有:
      Thermofisher旗下Gibco培养系列、ABI细胞计数仪及PCR仪、Invitrogen分子生物学试剂及Qubit3.0、Ambion的RNA相关试剂、Molecular probes试剂、Pierce蛋白相关试剂盒、Nanodrop One/OneC;Parafilm封口膜;Viskase透析袋;Sigma-Aldrich试剂;AccuStandard 标准品;Millipore抗体;Glen Research 核酸合成试剂;Bio-Rad电泳仪及PCR仪;Nanocs的PEG聚合物;  Chromotek羊驼抗体;   Polysciences转染试剂;  Echelon-inc 脂类研究试剂盒;   R&D Systems抗体及Elisa试剂盒; Jackson二抗及封闭血清; Biotium 荧光染料;NEB内切酶及NGS相关产品;  Athens Research & Technology人类蛋白及抗血清;  Illumina二代测试试剂盒; Tocris 小分子试剂; TwistDx恒温扩增试剂盒(RPA); Enzymatics二代测序酶;Novus抗体及Elisa试剂盒;AG scientific潮霉素B、G-418;Plantmedia植物培养系列及抗体;Agilent 2100生物分析仪;Biolog耗材;Biomatik的Elisa试剂盒;Covaris基因打断仪;CST抗体;Dharmacon的siRNA产品。

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