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invitrogen/A11029/A11034二抗现货促销
  • 发布日期:2017-12-29      浏览次数:537
    • ThermoFisherA11029Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
      ThermoFisherA11034Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate

      invitrogen/A11029/A11034二抗现货促销

      订货找蚂蚁淘就对了,4000-520-626

      Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11034) in IF

      Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891)。 The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0。1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Rabbit primary antibody for 3 hours at room temperature。 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate (Product # A-11034) was used at a concentration of 4 µg/ml in phosphate buffered saline containing 0。2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green)。 Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938)。 F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red)。 Panel d represents the composite image。 No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e)。 The images were captured at 60X magnification。

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