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Biomatrica公司AssayStable PCRstable
  • 发布日期:2018-03-27      浏览次数:334
    • Biomatrica公司AssayStable  PCRstable

      Biomatrica授权苏州蚂蚁淘生物科技秒速牛牛官网代理销售旗下产品,

      Biomatrica开发出一种新型的核心技术,旨在防止复杂的样品和试剂在运输、存储以及处理过程中的降解,从而使科学家们能获得更多重复性和可靠的实验结果,提率,节省时间。Biomatrica目前的产品稳定核酸并防止降解,而无需冷藏

       AssayStable® & PCRstable®
      Precise positioning and controlled release of assay reagents not achievable by lyophilization
      AssayStable and PCRstable are ambient temperature chemistries that allows assay manufacturers to precisely position, preserve, and release their assay reagents in any format using Biomatrica’s unique reagents with a simple air-dry process. PCRstable was developed for PCR-based assays, and AssayStable was developed for all other assays. 

      Overview
      Assay Manufacturers:
      Air-dry any assay reagent in-house and store at room temperature
      No lyophilization and no low humidity chambers needed
      Virtually any reagent stabilized by patented stabilizers
      Assay Users:
      Prepare any assay in a dry-stable format
      Add template and go; no mixing needed
      Rapid rehydration upon template addition

       Use Cases
      GenMark Diagnostics: Valve-Free Positioning and Preserving of Sample-to-Answer Diagnostic Devices
      Thermo Fisher Ion AmpliSeq: Preservation of High Throughput Next Generation Sequencing Arrays

      Focus Diagnostics: Enabling decentralized, point-of-care diagnostics through chemical biostabilization
      Extending shelf-life of one-step, liquid RT-PCR assays for point of care
      Instantaneous Preservation of Rare Cells in Blood to Evaluate Cancer Therapy Selection
      PCR Assay Mastermix Ambient Stabilization Without Lyophilization
      Stabilization of Multiplexed Panel for Next-Generation Sequencing 

      Product Data

      Stabilization of qPCR assay

      Included in dry-down:

      • Taq
      • Primers
      • Reverse Transcriptase
      • Dye (SYBR® Green)/Taqman®
      • dNTPs
      • Salts and Detergent

      Stabilization of qPCR assay at ambient temperature

      Figure 1: Components of qPCR assay, including Taq DNA polymerase, dNTPs, primers for RNase P, SYBR Green dye and PCR buffer were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C。 At determined time points, samples were rehydrated with water and MgCl2 with the specified amount of genomic DNA template。 Real time PCR amplification of a RNase P fragment was performed on the ABI 7300 Real-Time PCR System, using the equivalent amount of DNA with qPCR reagents stored at -20°C as a positive control。 Data shown is for samples stored for 6 months at 45°C, corresponding to 2 years of ambient temperature storage, based on accelerated aging。

      Stabilization of RT-qPCR assay at ambient temperature

      Figure 2: All components of a RT-qPCR assay, including Taq DNA polymerase with reverse transcriptase activity, dNTPs, aptamer and PCR buffer including metal co-factor were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C. At determined time points, samples were rehydrated with water, primers and TaqMan probes, and a known input of RNA template was added. Reverse transcription and real time PCR amplification were performed on ABI 7300 Real-Time PCR System, using the equivalent amount of RNA samples with RT-qPCR reagents stored at -20°C as a positive control. Data shown is for samples stored for 3 weeks at 45°C, corresponding to 3 months of ambient temperature storage, based on accelerated aging.

      qPCR Stabilized

      Figure 3: Taq + dNTPs stabilized for 4 years at room temperature(based on accelerated aging).

      Stabilization of a Limiting Quantity of Reverse Transcriptase

      Figure 4: Stabilization of a limiting amount of reverse transcriptase for rapid screening ability.

      Ambient Shipping: Stress Testing

      Figure 5: Biomatrica stabilizers protected all samples, while non-protected shows complete degradation below 10 ng.

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