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Flow Tech公司MICROFIL® 造影剂现货
  • 发布日期:2018-03-13      浏览次数:532
    • MICROFIL® Silicone Rubber Injection Compounds造影剂

      MV-112 MV-117 MV-120 MV-122 MV-130 MV-132 MV-Diluent

      http://www.flowtech-inc。。com

       

      About MICROFIL®

      MICROFIL® compounds will fill and opacify microvascular and other spaces of non-surviving animals and post-mortem tissue under physiological injection pressure. The continuous, closed vascular system lends itself to flow through injection or perfusion techniques. Following injection, MICROFIL compounds cure to form a three-dimensional cast of the vasculature.
      MICROFIL® MV-series compounds are available in five radiopaque colors, as well as clear. MV-series compounds require either an alcohol-methyl salicylate or glycerin clearing sequence, whereby the refractive index of the clearing solution is the same as the refractive index of the tissue. This allows for microscopic examination of a selected vascular bed.
      MICROFIL® CP-101 compound is intended for use in cast-corrosion techniques, and is designed for filling large blood vessels (greater than 100 microns). Although the CP-101 compound will fill capillaries, these vessels fragment when the supporting tissue is removed through exposure to a potassium hydroxide solution. When cured, MICROFIL® CP-101 is a milky white in color. Casts made using CP-101 will maintain their dimensional accuracy indefiniy.
      Advantages offered with MICROFIL® compounds over previously available rubber injection materials include:
      · Complete filling with minimal shrinkage, to enhance vessel continuity and to produce in cleared preparations a vivid, optically cleared specimen that allows a precise study of the microcirculation.
      · Color diversity, to provide delineation within the circulatory tree for microscopic examination and photographic illustration.

      Areas of Investigation

      In physiology, MICROFIL® visualization provides a means for establishing the precise vascular architecture of specific organs, allowing comparison between normal and abnormal structure。
      In surgery, visualization of the microcirculation and microanatomy is leading to improved surgical techniques in the repair of nerves, tendons, and blood vessels.
      In gastrointestinal research, MICROFIL® compounds characterize and describe changes in vascular patterns associated with several pathological conditions.
      Injected specimens, when preserved in methyl salicylate or glycerin, also serve as a definitive teaching adjunct.

      MV-Series Mixing Procedure

      To achieve a viscosity level suitable for injection of the microcirculation, it is necessary to blend the MV compound with an equal quantity (by weight) of MV-Diluent. Volume mixing requires 5ml of diluent for every 4ml of compound. The mixture of compound and diluent is catalyzed with 5% (by weight or volume) of MV Curing Agent. Viscosity ranges from 20 to 30 centipoise. Working time is 20 minutes and begins with the addition of curing agent.



       

      Notes

      1. Viscosity measured with a Brookfield Model LVF Viscometer, with a No. 2 spindle at 30RPM.
      2. Gel time measured on a blend (by weight) of one part MV compound and one part MV-Diluent followed by addition of 5% MV Curing Agent. Gel time is time required for mixture to cease flowing.
      3. Shelf life is in excess of four months. For materials held beyond four months, it is possible to run the following static test to determine acceptability:
      a. Mix 5 grams of MV compound with 5 grams of MV-Diluent in a vial.
      b。 Add 10 drops (medicine dropper) of MV Curing Agent。
      c. Cap, shake, and refrigerate overnight.
      d. The mixture should gel in the vial after this procedure to assure a cure in subsequent animal injections.
      Catalyzed mixtures will form an elastomeric gel after 90 minutes at room temperature. Curing takes place with non-exothermic cross-linking and minimal volume change.
      It has been possible to refrigerate specimens immediay after injection and still obtain complete cure after overnight aging. The procedure decreases odor level for subsequent sectioning.

      Perfusion Techniques

      Two techniques of tissue clearing are described below. Alcohol-methyl salicylate clearing produces a stiffer tissue, which, from an aesthetic point, provides a pleasing view for gross observation. Glycerin clearing produces a more flexible tissue, allowing easier manipulation for a given vessel.

      Alcohol-methyl salicylate clearing

      Non-wetting features of MICROFIL® compounds prevent any interaction with blood. Therefore, in the non-surviving animal, a selected vascular bed can be readily perfused without prior washout of blood. Heparinization to maintain blood fluidity, however, has been used to realize improved injection preparations.
      For the injection of blood vessels from vascular beds removed post-mortem, washout of clotted blood with saline is advisable.
      Selected vascular beds are perfused through their accessible artery and drained through a similar vein. Infusion pressures will vary with the animal’s mean systemic pressure. For organs from the dog, cat, rat, and man, a pressure of 150mm.Hg for arterial filling has been used, and 25 to 50mm.Hg for venous fillings.

      Flow Tech公司MICROFIL® 造影剂现货

      Flow Tech,Inc.是一家马萨诸塞州公司,于1996年从Boulder公司搬迁,我们能为您提供便利和专业的服务,在大多数情况下,您可以在收到后的一个工作日内发货。

      MICROFIL®化合物设计用于在生理压力下填充和遮盖非生存动物和尸体后的微血管和其他空间。这一创新产品已经在美国和国际ling先的高校,医院和其他研究机构使用了三十多年。

      关于MICROFIL®

      MICROFIL®化合物将在生理注射压力下填充和不透明非生存动物和死后组织的微血管和其他空间。连续闭合的血管系统本身可以通过注射或灌注技术流动。注射后,MICROFIL化合物固化形成脉管系统的三维铸型。

      MICROFIL®MV系列化合物有五种不透射线的颜色以及透明度。MV系列化合物需要水杨酸甲酯或甘油清除顺序,由此清除溶液的折射率与组织的折射率相同。这允许对所选择的血管床进行显微镜检查。

      MICROFIL®CP-101化合物适用于铸造腐蚀技术,用于填充大血管(大于100微米)。虽然CP-101化合物将填充毛细血管,但是当通过暴露于氢氧化钾溶液除去支持组织时,这些血管碎片。固化时,MICROFIL®CP-101是乳白色的白色。使用CP-101制作的铸件将无限期地保持其尺寸精度。

      MICROFIL®化合物优于以前提供的橡胶注射材料的优势包括:

      • 完全填充zui小的收缩,增强血管连续性,并在清除的制剂中产生一个生动的,光学清除的样品,允许研究微循环。
      • 色彩多样性,在循环树中提供用于显微镜检查和照相插图的描绘。

      Flow Tech公司MICROFIL® 造影剂现货

      Flow Tech公司MICROFIL® 造影剂现货

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