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21120-AAT Bioquest公司荧光探针Rhod-4™使用说明

供应数量:108

发布日期:2019/5/21

有效日期:2020/5/21

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AAT Bioquest公司荧光探针Rhod-4™使用说明

苏州蚂蚁淘生物科技秒速牛牛官网是AATBIO的中国大陆区授权代理商

美国AAT Bioquest Inc。(前身是ABD BioquestInc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。

1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物;

2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞;

3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类;

4)致力于开发用于信号转导研究的试剂;

5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。

AAT Bioquest公司荧光探针Rhod-4™使用说明

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge

 

Ex/Em (nm)524/551
MW1015.96
Kd (nM)451
SolventDMSO
StorageFreeze (<-15 °C)
Minimize light exposure
InstrumentsFluorescence microplate reader, Fluorescence microscope, Flow cytometer
CategoryGPCR
Calcium GPCR Assays
Related

Calcium Channels
pH and Ion Indicators
Secondary Reagents

 

 

Calculators
Common stock solution preparation 
 

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

 

 0.1 mg0.5 mg1 mg5 mg10 mg
1 mM98.429 µL492.145 µL984.291 µL4.921 mL9.843 mL
5 mM19.686 µL98.429 µL196.858 µL984.291 µL1.969 mL
10 mM9.843 µL49.215 µL98.429 µL492.145 µL984.291 µL
 
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